Peptide glossary · Analysis
ESI-MS
Mass spectrometry with electrospray ionisation. Peptides become multiply charged, so the spectrum shows several signals for the same molecule.
In the lab
The software converts the different charge states back to the neutral mass. A peptide of 1419.5 Da appears doubly charged at about m/z 710.8.
More terms: Analysis
- AggregationPeptide molecules clumping together, often with hydrophobic sequences.
- Amino acid analysisA method in which the peptide is fully hydrolysed and the individual amino acids are measured.
- Area percentThe unit of HPLC purity: the main peak's area divided by the area of all peaks.
- ChromatogramThe curve an HPLC run produces: detector signal over time.
- CounterionThe ion that balances the charge of a peptide salt, such as trifluoroacetate or acetate.
- DeamidationA degradation route in which asparagine or glutamine lose their amide group.
For laboratory research use only. Not for use in humans or animals.