Peptide glossary · Analysis
Aggregation
Peptide molecules clumping together, often with hydrophobic sequences. Aggregates make solutions cloudy, and the real concentration is then unknown.
In the lab
Hydrophobic peptides often dissolve only in organic solvent. The aqueous buffer is then added dropwise to the dissolved peptide, not the other way round.
Read moreDissolving peptides: solubility for in vitro workMore terms: Analysis
- Amino acid analysisA method in which the peptide is fully hydrolysed and the individual amino acids are measured.
- Area percentThe unit of HPLC purity: the main peak's area divided by the area of all peaks.
- ChromatogramThe curve an HPLC run produces: detector signal over time.
- CounterionThe ion that balances the charge of a peptide salt, such as trifluoroacetate or acetate.
- DeamidationA degradation route in which asparagine or glutamine lose their amide group.
- EndotoxinsCell wall components of certain bacteria that can strongly distort cell experiments.
For laboratory research use only. Not for use in humans or animals.