Peptide glossary · Analysis
TFA (trifluoroacetic acid)
A strong acid used in synthesis and HPLC purification. Peptides are then often present as trifluoroacetate salts. For cell work an exchange to acetate is sometimes wanted.
In the lab
A trifluoroacetate ion weighs about 114 g/mol. Exchanging it for acetate at about 60 g/mol raises the peptide content accordingly.
More terms: Analysis
- AggregationPeptide molecules clumping together, often with hydrophobic sequences.
- Amino acid analysisA method in which the peptide is fully hydrolysed and the individual amino acids are measured.
- Area percentThe unit of HPLC purity: the main peak's area divided by the area of all peaks.
- ChromatogramThe curve an HPLC run produces: detector signal over time.
- CounterionThe ion that balances the charge of a peptide salt, such as trifluoroacetate or acetate.
- DeamidationA degradation route in which asparagine or glutamine lose their amide group.
For laboratory research use only. Not for use in humans or animals.